Primer

Part:BBa_K2680514

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-07-30)


WM_Pad2 Construction primer

This primer along with BBa_K2680513 is designed to create the initial 1C3 iGEM backbone containing William and Mary launch pad sequences for use with level 0 parts.

Thermodynamic Info

No hairpin concerns
-11.96 kcal homodimer
-11.36 kcal heterodimer with BBa_K2680513
T_m in Q5 HiFi 2X MasterMix (from NEB): 55C
Anneal with BBa_K2680513 at 54C

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None